CD86 antibody (FITC)

Catalog No. ABIN316884

This Rat Monoclonal antibody specifically detects CD86 in FACS. It exhibits reactivity toward Mouse.

Quick Overview for CD86 antibody (FITC) (ABIN316884)

Target: CD86

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD86 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: PO3

Product Details anti-CD86 Antibody (FITC)

Specificity: This antibody recognises CD86 (B7-2). The PO3 clone has been reported to block some co-stimulatory functions of CD86.

Characteristics: Synonyms: CD28LG2, B70, FUN-1, BU63, T-lymphocyte activation antigen CD86, Activation B7-2antigen, CTLA-4 counter-receptor B7.2

Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Immunogen: Mouse B-cell line, BCL1.

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0,1 mg/mL

Buffer: Phosphate buffered saline pH 7.4 containing 0.09 % Sodium Azide, 1 % Bovine Serum Albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Shelf life: one year from despatch. Caution: (A full Health and Safety assessment is available upon request). This product containsSodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by

Expiry Date: 12 months

Target Details for CD86

Target: CD86

Alternative Name: CD86

Background: CD86 is an 80kD cell surface glycoprotein which is a member of the CD28/B7 family. In mouse, CD86 is expressed at high levels on peripheral blood monocytes, dendritic cells and at low levels on resting B and T-lymphocytes. Expression of CD86 on these cell populations can be increased upon activation. CD86 has been identified, along with CD80 (B7-1) as a ligand for CD28 and cytotoxic T-lymphocyte antigen-4 (CTLA4). CD28 and CTLA4 are two receptors that have opposing functions in T-cell stimulation. Interaction of CD86 with CD28 promotes a number of T-cell activities, whereas the binding of CD86 to CTLA4 inhibits T-cell responses.Synonyms: Activation B7-2 antigen, B7.2, B70, BU63, CD28LG2, CTLA-4 counter-receptor B7.2, FUN-1, T-lymphocyte activation antigen CD86

Gene ID: 12524

UniProt: P42082

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation